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Published online 《Theoretical and Applied Genetics》
 
 
Fine mapping of a gene for low-tiller number, Ltn, in japonica rice (Oryza sativa L.) variety Aikawa 1

Daisuke Fujita, Leodegario A. Ebron, Etsuko Araki, Hiroshi Kato, Gurdev S. Khush, John E. Sheehy, Tanguy Lafarge, Yoshimichi Fukuta and Nobuya Kobayashi

 
 

International Rice Research Institute, DAPO Box 7777, Metro Manila, Philippines

 
 
Abstract:Tillering is one of the most important agronomic traits related to grain production in rice (Oryza sativa L.). A japonica-type variety, Aikawa 1, is known to have low-tiller number. The detailed location of a low-tillering gene, Ltn, which has been localized on chromosome 8 in Aikawa 1, was confirmed by molecular mapping. Using BC5F2 individuals derived from a cross between IR64 and Aikawa 1, the low-tillering gene was mapped to an interval defined by SSR markers ssr5816-3 and A4765. This was designated as Ltn because there was no reported gene for tillering in the region of chromosome 8. Through high-resolution linkage analysis, the candidate region of Ltn was located between DNA markers ssr6049-23 and ind6049-1 corresponding to 38.6 kbp on the Nipponbare genome sequence. These DNA markers, which were tightly linked to Ltn, are useful for marker-assisted selection in breeding studies.
 
     
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